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Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1
http://hdl.handle.net/10487/5275
http://hdl.handle.net/10487/527567675b01-67ae-43af-998d-2d4059114a27
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
04 19 13-17.pdf (589.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2009-12-21 | |||||
| タイトル | ||||||
| タイトル | Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 3-hydroxybutyrate dehydrogenase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ralstonia pickettii | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | poly-3-hydroxybutyrate | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Takanashi, Masahiko
× Takanashi, Masahiko× Shiraki, Mari× 白木, 麻里× Saito, Terumi× 齊藤, 光實 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We previously reported that Ralstonia pickettii T1, a bacterium growing on extracellular poly-3-hydroxybutyrate (PHB), have two 3-hydroxybutyrate dehydrogenases (BDH1 and BDH2). By analysis of knockout mutants of bdh1 or bdh2 using anionexchange column chromatography, it was shown that a novel BDH besides BDH1 and BDH2 was present in R. pickettii T1. The third BDH (BDH3) was partially purified by column chromatography, and the enzyme had the N-terminal amino acid sequence different from those of BDH1 and BDH2. In Southern blotting with bdh2 as a probe, bdh3 was detected and cloned, and the purified gene product of bdh3 expressed in Escherichia coli showed higher specific activity than those of BDH1 and BDH2. |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ■Full-Length Paper■ By a grand of Research Institute for Integral Science, Kanagawa University | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 19, p. 13-17, 発行日 2008-06-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
