@article{oai:kanagawa-u.repo.nii.ac.jp:00006958,
 author = {Takanashi, Masahiko and Shiraki, Mari and 白木, 麻里 and Saito, Terumi and 齊藤, 光實},
 journal = {Science Journal of Kanagawa University},
 month = {Jun},
 note = {We previously reported that Ralstonia pickettii T1, a bacterium growing on
extracellular poly-3-hydroxybutyrate (PHB), have two 3-hydroxybutyrate dehydrogenases
(BDH1 and BDH2). By analysis of knockout mutants of bdh1 or bdh2 using anionexchange
column chromatography, it was shown that a novel BDH besides BDH1 and BDH2
was present in R. pickettii T1. The third BDH (BDH3) was partially purified by column
chromatography, and the enzyme had the N-terminal amino acid sequence different from
those of BDH1 and BDH2. In Southern blotting with bdh2 as a probe, bdh3 was detected
and cloned, and the purified gene product of bdh3 expressed in Escherichia coli showed
higher specific activity than those of BDH1 and BDH2., ■Full-Length Paper■ By a grand of Research Institute for Integral Science, Kanagawa University},
 pages = {13--17},
 title = {Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1},
 volume = {19},
 year = {2008}
}