{"created":"2023-05-15T11:46:25.287834+00:00","id":6958,"links":{},"metadata":{"_buckets":{"deposit":"94e0cf6a-3c77-4f05-94ca-80ee4617c857"},"_deposit":{"created_by":1,"id":"6958","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6958"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006958","sets":["376:377:378:558"]},"author_link":["20924","20923","20920","20922","20921"],"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"17","bibliographicPageStart":"13","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously reported that Ralstonia pickettii T1, a bacterium growing on\nextracellular poly-3-hydroxybutyrate (PHB), have two 3-hydroxybutyrate dehydrogenases\n(BDH1 and BDH2). By analysis of knockout mutants of bdh1 or bdh2 using anionexchange\ncolumn chromatography, it was shown that a novel BDH besides BDH1 and BDH2\nwas present in R. pickettii T1. The third BDH (BDH3) was partially purified by column\nchromatography, and the enzyme had the N-terminal amino acid sequence different from\nthose of BDH1 and BDH2. In Southern blotting with bdh2 as a probe, bdh3 was detected\nand cloned, and the purified gene product of bdh3 expressed in Escherichia coli showed\nhigher specific activity than those of BDH1 and BDH2.","subitem_description_type":"Abstract"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"■Full-Length Paper■ By a grand of Research Institute for Integral Science, Kanagawa University","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takanashi, Masahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiraki, Mari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"白木, 麻里"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Terumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"齊藤, 光實"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"04 19 13-17.pdf","filesize":[{"value":"589.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"04 19 13-17.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6958/files/04 19 13-17.pdf"},"version_id":"1460829c-104d-4dfb-9d4f-c2604cd7a592"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"3-hydroxybutyrate dehydrogenase","subitem_subject_scheme":"Other"},{"subitem_subject":"Ralstonia pickettii","subitem_subject_scheme":"Other"},{"subitem_subject":"poly-3-hydroxybutyrate","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1"}]},"item_type_id":"3","owner":"1","path":["558"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-21"},"publish_date":"2009-12-21","publish_status":"0","recid":"6958","relation_version_is_last":true,"title":["Characterization of Third 3-Hydroxybutyrate Dehydrogenase in Ralstonia pickettii T1"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:44:44.867573+00:00"}