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クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定
http://hdl.handle.net/10487/00015999
http://hdl.handle.net/10487/0001599919ae935b-55c9-409a-bfe3-eab84e27bff6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10 クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定 (1.5 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-18 | |||||
| タイトル | ||||||
| タイトル | クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Purification of Crustacean Female Sex Hormone from the Eyestalk of the Kuruma Prawn Marsupenaeus japonicus and Its Biological Activity | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | kuruma prawn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Marsupenaeus japonicus | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | crustacean female sex hormone (CFSH) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | vitellogenesis-inhibiting hormones (VIH) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | kuruma prawn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Marsupenaeus japonicus | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | crustacean female sex hormone (CFSH) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | vitellogenesis-inhibiting hormones (VIH) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
安保, 裕子
× 安保, 裕子× 渡部, 聡美× 甲髙, 彩華× 大平, 剛 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Recently, crustacean female sex hormone (CFSH) has been purified from the female eyestalk of the crab Callinectes sapidus. Gene knockdown of C. sapidus CFSH by RNA interference was shown to inhibit the appearance of female reproductive characteristics. This result suggests that CFSH controls female secondary sex characteristics. Recently, we cloned cDNA encoding the Kuruma prawn Marsupenaeus japonicus CFSH (Maj-CFSH). In order to examine the biological function of Maj-CFSH, we purified native Maj-CFSH and assessed its biological function. In our previous study, native Maj-CFSH was localized in the sinus gland located in the eyestalk of M. japonicus. Therefore, sinus glands were dissected under a stereo microscope and an extract was subsequently prepared. The sinus gland extract was subjected to reverse-phase HPLC. All peak fractions were collected and subjected to western blot analysis using anti-Maj-CFSH antibody. As a result, immunopositive band was detected only in a single peak fraction. This peak fraction was subjected to N-terminal amino acid sequence analysis and then 10 N-terminal amino acid residues were determined. This N-terminal sequence was identical to that of the mature Maj-CFSH. These results indicate that we have successfully purified native Maj-CFSH. The effect of the purified native Maj-CFSH on vitellogenin (VG) gene expression was examined using an ex vivo ovarian incubation system. Maj-CFSH had an inhibitory effect on VG gene expression, whereas the negative control (medium only) did not. Maj-SGP-VII, which is one of six vitellogenesis-inhibiting hormones (VIH) in M. japonicus, also had a clear inhibitory effect on VG gene expression levels in this incubation system. These results suggest that Maj-CFSH exhibits vitellogenesis-inhibiting activity in M. japonicus. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 原著 | |||||
| 書誌情報 |
en : Science Journal of Kanagawa University 巻 30, p. 63-66, 発行日 2019-06-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Purification of Crustacean Female Sex Hormone from the Eyestalk of the Kuruma Prawn Marsupenaeus japonicus and Its Biological Activity | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学総合理学研究所 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Departmental Bulletin Paper | |||||
