{"created":"2023-05-15T11:51:03.862831+00:00","id":12908,"links":{},"metadata":{"_buckets":{"deposit":"0bfd3834-b091-4d52-9a88-bf05e45f2eb7"},"_deposit":{"created_by":10,"id":"12908","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"12908"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00012908","sets":["376:377:378:1252"]},"author_link":["34336","34337","34338","33616","34333","34334","34335","33616"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Purification of Crustacean Female Sex Hormone from the Eyestalk of the Kuruma Prawn Marsupenaeus japonicus and Its Biological Activity"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"66","bibliographicPageStart":"63","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recently, crustacean female sex hormone (CFSH) has been purified from the female eyestalk of the crab Callinectes sapidus. Gene knockdown of C. sapidus CFSH by RNA interference was shown to inhibit the appearance of female reproductive characteristics. This result suggests that CFSH controls female secondary sex characteristics. Recently, we cloned cDNA encoding the Kuruma prawn Marsupenaeus japonicus CFSH (Maj-CFSH). In order to examine the biological function of Maj-CFSH, we purified native Maj-CFSH and assessed its biological function. In our previous study, native Maj-CFSH was localized in the sinus gland located in the eyestalk of M. japonicus. Therefore, sinus glands were dissected under a stereo microscope and an extract was subsequently prepared. The sinus gland extract was subjected to reverse-phase HPLC. All peak fractions were collected and subjected to western blot analysis using anti-Maj-CFSH antibody. As a result, immunopositive band was detected only in a single peak fraction. This peak fraction was subjected to N-terminal amino acid sequence analysis and then 10 N-terminal amino acid residues were determined. This N-terminal sequence was identical to that of the mature Maj-CFSH. These results indicate that we have successfully purified native Maj-CFSH. The effect of the purified native Maj-CFSH on vitellogenin (VG) gene expression was examined using an ex vivo ovarian incubation system. Maj-CFSH had an inhibitory effect on VG gene expression, whereas the negative control (medium only) did not. Maj-SGP-VII, which is one of six vitellogenesis-inhibiting hormones (VIH) in M. japonicus, also had a clear inhibitory effect on VG gene expression levels in this incubation system. These results suggest that Maj-CFSH exhibits vitellogenesis-inhibiting activity in M. japonicus.","subitem_description_type":"Abstract"}]},"item_3_description_40":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Departmental Bulletin Paper","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学総合理学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安保, 裕子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"渡部, 聡美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"甲髙, 彩華"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大平, 剛"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-18"}],"displaytype":"detail","filename":"10 クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10 クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定","url":"https://kanagawa-u.repo.nii.ac.jp/record/12908/files/10 クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定.pdf"},"version_id":"7831caa3-44af-4297-8784-bd9c06577031"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"kuruma prawn","subitem_subject_scheme":"Other"},{"subitem_subject":"Marsupenaeus japonicus","subitem_subject_scheme":"Other"},{"subitem_subject":"crustacean female sex hormone (CFSH)","subitem_subject_scheme":"Other"},{"subitem_subject":"vitellogenesis-inhibiting hormones (VIH)","subitem_subject_scheme":"Other"},{"subitem_subject":"kuruma prawn","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Marsupenaeus japonicus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crustacean female sex hormone (CFSH)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vitellogenesis-inhibiting hormones (VIH)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定","subitem_title_language":"ja"},{"subitem_title":"Purification of Crustacean Female Sex Hormone from the Eyestalk of the Kuruma Prawn Marsupenaeus japonicus and Its Biological Activity","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["1252"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-18"},"publish_date":"2020-03-18","publish_status":"0","recid":"12908","relation_version_is_last":true,"title":["クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-06-19T02:05:34.284288+00:00"}