@article{oai:kanagawa-u.repo.nii.ac.jp:00012908,
 author = {安保, 裕子 and 渡部, 聡美 and 甲髙, 彩華 and 大平, 剛},
 journal = {Science Journal of Kanagawa University},
 month = {Jun},
 note = {Recently, crustacean female sex hormone (CFSH) has been purified from the female eyestalk of the crab Callinectes sapidus. Gene knockdown of C. sapidus CFSH by RNA interference was shown to inhibit the appearance of female reproductive characteristics. This result suggests that CFSH controls female secondary sex characteristics. Recently, we cloned cDNA encoding the Kuruma prawn Marsupenaeus japonicus CFSH (Maj-CFSH). In order to examine the biological function of Maj-CFSH, we purified native Maj-CFSH and assessed its biological function. In our previous study, native Maj-CFSH was localized in the sinus gland located in the eyestalk of M. japonicus. Therefore, sinus glands were dissected under a stereo microscope and an extract was subsequently prepared. The sinus gland extract was subjected to reverse-phase HPLC. All peak fractions were collected and subjected to western blot analysis using anti-Maj-CFSH antibody. As a result, immunopositive band was detected only in a single peak fraction. This peak fraction was subjected to N-terminal amino acid sequence analysis and then 10 N-terminal amino acid residues were determined. This N-terminal sequence was identical to that of the mature Maj-CFSH. These results indicate that we have successfully purified native Maj-CFSH. The effect of the purified native Maj-CFSH on vitellogenin (VG) gene expression was examined using an ex vivo ovarian incubation system. Maj-CFSH had an inhibitory effect on VG gene expression, whereas the negative control (medium only) did not. Maj-SGP-VII, which is one of six vitellogenesis-inhibiting hormones (VIH) in M. japonicus, also had a clear inhibitory effect on VG gene expression levels in this incubation system. These results suggest that Maj-CFSH exhibits vitellogenesis-inhibiting activity in M. japonicus., Departmental Bulletin Paper, 原著},
 pages = {63--66},
 title = {クルマエビ眼柄甲殻類雌性ホルモンの精製と生物活性の測定},
 volume = {30},
 year = {2019}
}