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An Alternatively Spliced Site in the Proline-Rich Region of Microtubule–Associated Protein 4 Plays an Important Role in Microtubule Stabilization
http://hdl.handle.net/10487/5277
http://hdl.handle.net/10487/527774ea9b48-6379-429d-8f7e-6f49f7a4ff87
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
03 19 7-11.pdf (874.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2009-12-21 | |||||
| タイトル | ||||||
| タイトル | An Alternatively Spliced Site in the Proline-Rich Region of Microtubule–Associated Protein 4 Plays an Important Role in Microtubule Stabilization | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pro-rich region | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule-associated protein 4 (MAP4) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Tokuraku, Kiyotaka
× Tokuraku, Kiyotaka× Moriyama, Hatsune× Matsushima, Kazuyuki× Kotani, Susumu× 小谷, 享 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We previously reported that the microtubule-stabilizing activity of a microtubule-associated protein (MAP) 4 variant, with a deletion in the Pro-rich region (MAP4-SP), was lower than that of a variant with a full length Pro-rich region (MAP4-LP). However, it remained unclear whether the deletion of the specific site in the Pro-rich region is responsible for the reduction of the microtubule-stabilizing activity. To answer this question, we examined the microtubule-stabilizing activities of four different MAP4 variants, MAP4-SP, MAP4-LP, and two additional MAP4-LP variants lacking a part of the Repeat region, and considered the correlation between the activity and the structure. When microtubules assembled in the presence of each of the MAP4 variants were treated with nocodazole for disassembly, the MAP4-SP-induced microtubules were significantly less stable than the other variant-induced microtubules. Another set of experiments, in which the microtubules were allowed to disassemble by dilution, yielded similar results: the MAP4-SP-induced microtubules were significantly less stable than the other variant-induced microtubules. The results clearly indicated that the microtubule-stabilizing activity of MAP4 depends on the specific, alternatively spliced site in the Pro-rich region. |
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| 書誌情報 |
Science Journal of Kanagawa University 巻 19, p. 7-11, 発行日 2008-06-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
