@article{oai:kanagawa-u.repo.nii.ac.jp:00006957,
 author = {Tokuraku, Kiyotaka and Moriyama, Hatsune and Matsushima, Kazuyuki and Kotani, Susumu and 小谷, 享},
 journal = {Science Journal of Kanagawa University},
 month = {Jun},
 note = {We previously reported that the microtubule-stabilizing activity of a
microtubule-associated protein (MAP) 4 variant, with a deletion in the Pro-rich region
(MAP4-SP), was lower than that of a variant with a full length Pro-rich region (MAP4-LP).
However, it remained unclear whether the deletion of the specific site in the Pro-rich region
is responsible for the reduction of the microtubule-stabilizing activity. To answer this
question, we examined the microtubule-stabilizing activities of four different MAP4
variants, MAP4-SP, MAP4-LP, and two additional MAP4-LP variants lacking a part of the
Repeat region, and considered the correlation between the activity and the structure.
When microtubules assembled in the presence of each of the MAP4 variants were treated
with nocodazole for disassembly, the MAP4-SP-induced microtubules were significantly less
stable than the other variant-induced microtubules. Another set of experiments, in which
the microtubules were allowed to disassemble by dilution, yielded similar results: the
MAP4-SP-induced microtubules were significantly less stable than the other variant-induced
microtubules. The results clearly indicated that the microtubule-stabilizing activity of
MAP4 depends on the specific, alternatively spliced site in the Pro-rich region.},
 pages = {7--11},
 title = {An Alternatively Spliced Site in the Proline-Rich Region of Microtubule–Associated Protein 4 Plays an Important Role in Microtubule Stabilization},
 volume = {19},
 year = {2008}
}