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An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells
http://hdl.handle.net/10487/1462
http://hdl.handle.net/10487/146295cc4023-eadc-443b-a5ab-276c6a0ee092
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SJKU Vol 18 11-17.pdf (730.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-23 | |||||
| タイトル | ||||||
| タイトル | An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | poly (3-hydroxybutyrate) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PHB | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | enzyme purification | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | intracellular PHB depolymerase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ralstonia eutropha H16 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Abe, Tomoko
× Abe, Tomoko× 安部, 智子× Kobayashi, Teruyuki× 小林, 照幸× Saito, Terumi× 齊藤, 光實 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A poly (3-hydroxybutyrate) (PHB) depolymerase was purified 4, 400-fold from the supernatant fraction of Ralstonia eutropha cells through fractionation with ammonium sulfate and chromatography with Toyopearl DEAE, Q Sepharose, and Toyopearl Ether. The partially purified preparation showed a high level of specific activity to hydrolyze amorphous PHB, comparable to PhaZd from R. eutropha. The degradation was inhibited by diisopropylphosphate (10mM) and dithiothreitol (100mM). Most of the degradation products were 3-hydroxybutyrate oligomers. The properties of the enzyme closely resembled those of PhaZd, but some differences were observed. This distinction from PhaZd was discussed. | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 18, p. 11-17, 発行日 2007-05-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
