{"created":"2023-05-15T11:46:24.389493+00:00","id":6938,"links":{},"metadata":{"_buckets":{"deposit":"e37c99b0-bf29-4047-a353-798965025412"},"_deposit":{"created_by":1,"id":"6938","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6938"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006938","sets":["376:377:378:557"]},"author_link":["20819","20823","20824","20820","20822","20821"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"17","bibliographicPageStart":"11","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A poly (3-hydroxybutyrate) (PHB) depolymerase was purified 4, 400-fold from the supernatant fraction of Ralstonia eutropha cells through fractionation with ammonium sulfate and chromatography with Toyopearl DEAE, Q Sepharose, and Toyopearl Ether. The partially purified preparation showed a high level of specific activity to hydrolyze amorphous PHB, comparable to PhaZd from R. eutropha. The degradation was inhibited by diisopropylphosphate (10mM) and dithiothreitol (100mM). Most of the degradation products were 3-hydroxybutyrate oligomers. The properties of the enzyme closely resembled those of PhaZd, but some differences were observed. This distinction from PhaZd was discussed.","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abe, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安部, 智子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Teruyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 照幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Terumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"齊藤, 光實"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"SJKU Vol 18 11-17.pdf","filesize":[{"value":"730.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SJKU Vol 18 11-17.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6938/files/SJKU Vol 18 11-17.pdf"},"version_id":"2054e0c6-8df5-419d-bdca-2e507f1926e0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"poly (3-hydroxybutyrate)","subitem_subject_scheme":"Other"},{"subitem_subject":"PHB","subitem_subject_scheme":"Other"},{"subitem_subject":"enzyme purification","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular PHB depolymerase","subitem_subject_scheme":"Other"},{"subitem_subject":"Ralstonia eutropha H16","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells"}]},"item_type_id":"3","owner":"1","path":["557"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-23"},"publish_date":"2009-02-23","publish_status":"0","recid":"6938","relation_version_is_last":true,"title":["An Intracellular PHB Depolymerase from the Supernatant Fraction of Ralstonia eutropha H16 Cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:45:07.549141+00:00"}