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扁形動物の微小管結合蛋白質の同定
https://doi.org/10.24792/0002001004
https://doi.org/10.24792/0002001004c0abc035-e351-4dd7-957b-36f389770343
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
05 扁形動物の微小管結合蛋白質の同定.pdf (2.5 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
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| 公開日 | 2024-09-20 | |||||||
| タイトル | ||||||||
| タイトル | 扁形動物の微小管結合蛋白質の同定 | |||||||
| 言語 | ja | |||||||
| タイトル | ||||||||
| タイトル | Identification of microtubule-binding proteins in flatworms | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | microtubule-associated proteins (MAPs) | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | assembly-promoting (AP) Sequence | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | tail | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | flatworm | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24792/0002001004 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
ミン, イゴウ
× ミン, イゴウ
× 越智, 拓海× 小谷, 享 |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Microtubule-associated proteins (MAPs) interact with microtubules and regulate cell division, cell morphogenesis, cell motility, spindle formation, and intracellular transport. Mammalian MAPs, MAP2, MAP4, and tau, have a repeat of the assembly-promoting (AP) sequence at the center of their microtubule-associating sites that bind directly to microtubules. Recently, genetic analysis revealed that molecules with repeating AP sequences exist throughout the animal kingdom, but it remains unclear whether these molecules function as MAPs. The purpose of this study was to identify primitive MAP molecules and examine species of the universality of their function in the animal kingdom using a primitive bilateria, the flatworm. First, protein expression vectors were created based on Stylochoplana pusilla MAPs (SpMAP_4R and SpMAP_5R) cDNA that differs in the number of AP sequence repeats. Next, we cloned the MAP-like gene of Dugesia ryukyuensis, which belongs to the same phylum of flatworms as Stylochoplana pusilla . Finally, the protein structures were compared based on the predicted amino acid sequences of SpMAPs and Dugesia MAPs. We found that the tail site of MAPs, which is present in Dugesia MAP-like protein and mammalian MAPs, is absent in SpMAPs. It has been reported that the tail site of MAPs is involved in microtubule polymerization and stability, but the details are unknown. Future studies of the functions MAPs in Stylochoplana pusilla and Dugesia might clarify the effect of the presence or absence of the tail site on microtubule polymerization. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 原著 2023年度神奈川大学総合理学研究所共同研究助成論文 | |||||||
| 言語 | ja | |||||||
| 書誌情報 |
ja : Science Journal of Kanagawa University en : Science Journal of Kanagawa University 巻 35, p. 25-30, 発行日 2023-07-30 |
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| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 1880-0483 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12068302 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 出版者 | ||||||||
| 出版者 | 神奈川大学総合理学研究所 | |||||||
| 言語 | ja | |||||||
| 出版者 | ||||||||
| 出版者 | Research Institute for Integrated Science, Kanagawa University | |||||||
| 言語 | en | |||||||
