{"created":"2024-09-20T00:17:16.023976+00:00","id":2001004,"links":{},"metadata":{"_buckets":{"deposit":"50ff7a93-0fe5-4a0a-a66d-bdd87839b8df"},"_deposit":{"created_by":10,"id":"2001004","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2001004"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:02001004","sets":["376:377:378:1726789902848"]},"author_link":["36190","34315"],"control_number":"2001004","item_3_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-07-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"30","bibliographicPageStart":"25","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"ja"},{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Microtubule-associated proteins (MAPs) interact with microtubules and regulate cell division, cell morphogenesis, cell motility, spindle formation, and intracellular transport. Mammalian MAPs, MAP2, MAP4, and tau, have a repeat of the assembly-promoting (AP) sequence at the center of their microtubule-associating sites that bind directly to microtubules. Recently, genetic analysis revealed that molecules with repeating AP sequences exist throughout the animal kingdom, but it remains unclear whether these molecules function as MAPs. The purpose of this study was to identify primitive MAP molecules and examine species of the universality of their function in the animal kingdom using a primitive bilateria, the flatworm. First, protein expression vectors were created based on Stylochoplana pusilla MAPs (SpMAP_4R and SpMAP_5R) cDNA that differs in the number of AP sequence repeats. Next, we cloned the MAP-like gene of Dugesia ryukyuensis, which belongs to the same phylum of flatworms as Stylochoplana pusilla . Finally, the protein structures were compared based on the predicted amino acid sequences of SpMAPs and Dugesia MAPs. We found that the tail site of MAPs, which is present in Dugesia MAP-like protein and mammalian MAPs, is absent in SpMAPs. It has been reported that the tail site of MAPs is involved in microtubule polymerization and stability, but the details are unknown. Future studies of the functions MAPs in Stylochoplana pusilla and Dugesia might clarify the effect of the presence or absence of the tail site on microtubule polymerization.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"原著　2023年度神奈川大学総合理学研究所共同研究助成論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_registration":{"attribute_name":"identifier_registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24792/0002001004","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学総合理学研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Research Institute for Integrated Science, Kanagawa University","subitem_publisher_language":"en"}]},"item_3_source_id_10":{"attribute_name":"item_3_source_id_10","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"item_3_source_id_8","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"PISSN"}]},"item_3_version_type_16":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ミン, イゴウ","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"越智, 拓海","creatorNameLang":"ja"},{"creatorName":"オチ, タクミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"36190","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小谷, 享","creatorNameLang":"ja"},{"creatorName":"コタニ, ススム","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"34315","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"34315","nameIdentifierScheme":"ローカル著者ID"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"05 扁形動物の微小管結合蛋白質の同定.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kanagawa-u.repo.nii.ac.jp/record/2001004/files/05 扁形動物の微小管結合蛋白質の同定.pdf"},"version_id":"1db75952-28b1-47a7-ac8d-f09c022360a2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microtubule-associated proteins (MAPs)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"assembly-promoting (AP) Sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tail","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"flatworm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"扁形動物の微小管結合蛋白質の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"扁形動物の微小管結合蛋白質の同定","subitem_title_language":"ja"},{"subitem_title":"Identification of microtubule-binding proteins in flatworms","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["1726789902848"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-09-20"},"publish_date":"2024-09-20","publish_status":"0","recid":"2001004","relation_version_is_last":true,"title":["扁形動物の微小管結合蛋白質の同定"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-10-09T06:33:44.446759+00:00"}