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走査型電子顕微鏡による花粉観察のための最適処理法の検討
http://hdl.handle.net/10487/5280
http://hdl.handle.net/10487/52803b31cd41-2398-42c9-abcc-43a6c543611a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
15 19 81-86.pdf (2.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-12-21 | |||||
| タイトル | ||||||
| タイトル | 走査型電子顕微鏡による花粉観察のための最適処理法の検討 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pollen | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Arabidopsis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | lily | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | preparation methods | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | scanning electron microscopyrs_j | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
重田, 英子
× 重田, 英子× Shigeta, Eiko× 岩元, 明敏× Iwamoto, Akitoshi× 風間, 真× Kazama, Makoto× 鈴木, 季直× Suzuki, Suechika |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To examine the most suitable method to prepare pollen for scanning electron microscope observation, several fixation methods and dehydration (or drying) methods were applied to Arabidopsis and lily pollen obtained from flowers before and after flowering. Light and confocal laser microscope observations revealed that, in both plants, pollen obtained before flowering was swollen and wet, while that obtained after flowering was shrunken and dry. For scanning electron microscopy, pollen was unfixed or fixed with 50% (eventually 100%) acetone, FAA (5% formalin with 50% ethanol and 5% acetic acid) or 6% glutaraldehyde solutions. It was then dried naturally in air or artificially by either critical point-dry or freeze-dry machines, respectively. The results indicated that, for scanning electron microscope observation of dry pollen, the most suitable treatment is natural drying without fixation. On the other hand, to observe wet pollen, the combination of FAA or glutaraldehyde fixation with artificial drying using either machines is preferable, and fixation with acetone is unsuitable. |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | テクニカルノート | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 19, p. 81-86, 発行日 2008-06-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Examination of the Most Suitable Preparation Method for Pollen Observation by Scanning Electron Microscope | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
