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Detection of the Centrosomal DNA from the Sperm of Bryophyte Marchantia polymorpha L. and Physcomitrella patens
http://hdl.handle.net/10487/1468
http://hdl.handle.net/10487/1468a38952ac-5d48-4fe7-8d28-ad2d3c154e03
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kana-2-3-0005 (832.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-23 | |||||
| タイトル | ||||||
| タイトル | Detection of the Centrosomal DNA from the Sperm of Bryophyte Marchantia polymorpha L. and Physcomitrella patens | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | centrosome | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | centrosomal DNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | S1 nuclease | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bryophyte sperm | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | liverwort | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Kawai, Shinobu
× Kawai, Shinobu× 河合, 忍× Watai, Erika× Sano, Takashi× Inoue, Kazuhito× 井上, 和仁× Hino, Akiya× 日野, 晶也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The liverwort Marchantia polymorpha is dioecious and haploid during most of its life cycle. It is known that fertilization in bryophytes, as in animals, involves sperm and eggs. Recently, we isolated novel DNA from the sperm centrosome of the starfish Asterina pectinifera. In the present study, we examined whether the sperm of bryophytes contain this unique DNA. The total DNA in the sperm of M. polymorpha was used as a template for polymerase chain reaction (PCR) analysis performed using a centriole-specific DNA primer. Although this primer was designed to specifically amplify the centrosomal DNA of the starfish, it can amplify the centrosomal DNA of many other animals having a similar DNA sequence. The size of the amplified PCR products was approximately 500 bp, which is similar to that of the PCR products amplified from starfish. We determined the nucleotide sequence similarities between the PCR products amplified from the cultured cells of the moss Physcomitrella patens and from the sperm of the liverwort M. polymorpha. Our results show that DNA similar to that of the starfish centrosomal DNA exists in bryophytes and includes animal centrosomal DNA homologues and candidate centrosomal DNA. | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 18, p. 19-25, 発行日 2007-05-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
