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Functional Analyses of a Neural Cell Specific Variant of Microtubule-Associated Protein 4
http://hdl.handle.net/10487/1443
http://hdl.handle.net/10487/1443b40854b6-5f02-4c54-96ab-9e719c6c873f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SJKU Vol 17 03-11.pdf (822.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2009-02-23 | |||||
| タイトル | ||||||
| タイトル | Functional Analyses of a Neural Cell Specific Variant of Microtubule-Associated Protein 4 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule-associated protein 4 (MAP4) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pro-rich region | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | neural MAP4 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule bundling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | septin2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microtubule stability and dynamics | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Hasan, Mohammad Rubayet
× Hasan, Mohammad Rubayet× Matsushima, Kazuyuki× Yue, Jin Ming× Nakagawa, Hiroyuki× 中川, 裕之× Miyamoto, Shigeaki× 宮本, 茂昭× Kotani, Susumu× 小谷, 享 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present study was conducted to analyze the functions of a recently reported neural cell specific variant of MAP4 (MAP4-SP) that lacks 72 consecutive amino acid residues in a region that is rich in proline and basic residues (pro-rich region). Although our previous study (Matsushima et al., 2005)^<12)>, using the microtubule-binding domains of the isoform and wild type MAP4 (MAP4-LP), demonstrated a difference in the microtubule bundling activity of the two proteins, here, using the full-length forms of the MAP4 proteins, we show that the proteins do not differ in their microtubule bundling activity both in vitro and in vivo. Expression of the MAP4 proteins, as C-terminal fusions to green fluorescent protein (GFP), in neuroblastoma cells revealed that MAP4-SP decorated microtubules were more remarkable in appearance than MAP4-LP decorated microtubules in the neuronal growth cones. Moreover, a microtubule destabilizing protein, septin2, which interacts with the pro-rich region of MAP4, was more active in destabilizing MAP4-SP-microtubules than MAP4-LP-microtubules in vitro. The susceptibility of MAP4-SP microtubules to destabilization by septin could be attributed to the weaker binding affinity of MAP4-SP for microtubules, as was reported earlier. Taken together, the current findings suggest the possibility that the neural MAP4, with its short pro-rich region, could be important in maintaining more dynamic microtubules in neural cells, and thus allowing more plasticity in and rapid morphological changes of these cells. | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 17, p. 3-11, 発行日 2006-05-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
