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Fluorescent in situ hybridization(FISH)法によるシロイヌナズナの花粉母細胞での減数第一分裂期染色体の動態解析(2003-2004年度神奈川大学共同研究奨励助成論文)
http://hdl.handle.net/10487/1425
http://hdl.handle.net/10487/1425a8215bf3-94c2-45d7-9d87-df1ce371790b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SJKU Vol 16 2005 25-33.pdf (1.5 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-23 | |||||
| タイトル | ||||||
| タイトル | Fluorescent in situ hybridization(FISH)法によるシロイヌナズナの花粉母細胞での減数第一分裂期染色体の動態解析(2003-2004年度神奈川大学共同研究奨励助成論文) | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | meiosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Arabidopsis thaliana | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | homologous chromosome | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | FISH(fluorescent in situ hybridization) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
安積, 良隆
× 安積, 良隆× Azumi, Yoshitaka× 外山, 俊土× Toyama, Takashi× 中村, 美奈子× Nakamura, Minako× 豊泉, 龍児× Toyoizumi, Ryuji× 風間, 真× Kazama, Makoto× 朝倉, 史明× Asakura, Nobuaki× 日野, 晶也× Hino, Akiya× 鈴木, 秀徳× Suzuki, Hideho |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Since insertion mutagenesis methods, which enabled us to identify the mutagenized genes routinely, were developed for plants, Arabidopsis thaliana has been playing a central role in plant meiosis research. Though several techniques to analyze meiotic chromosome behavior have been introduced into Arabidopsis research since Ross et al. reported the method to observe male meiotic chromosomes of this plant through light microscope in 1996 (Chromosome Res. 4-507-516), intimate analysis of the chromosome behavior has not been accomplished. Taking advantage of the recent development of new nucleotides labeled with fluorescent dyes, we investigated chromosome behavior during male meiosis by multicolor FISH. Telomeres found around nucleoli in premeiotic interphase cells dispersed after entering meiosis, then clustered in a bouquet-like configuration. Statistically, telomeres of homologous chromosomes paired earlier than centromeres, but when respective chromosomes were examined, the telomeres were not always quick to pair. At early prophase I, possibly at around the zygotene stage, the signals from telomeres reduced to less than ten. This reduction suggests that the paired telomeres of homologous chromosomes temporally associate with other telomeres to look for their real partners. When homologous chromosomes separated at anaphase I, telomeres were always last to segregate. This suggested that there was unknown interaction between the telomeres of homologs, connecting them until anaphase I started. | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 16, p. 25-33, 発行日 2005-05-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Analysis of Chromosome Dynamics during Meiosis I of Arabidopsis thaliana Pollen Mother Cells by Fluorescent In Situ Hybridization(FISH) | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
