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両生類アフリカツメガエルの心臓の形態形成におけるActomyosinの役割
http://hdl.handle.net/10487/00018155
http://hdl.handle.net/10487/0001815564df6b3f-d63c-4733-9981-8c3129bd0d8b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
09 両生類アフリカツメガエルの心臓の形態形成におけるActomyosinの役割 (4.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2022-09-20 | |||||
| タイトル | ||||||
| タイトル | 両生類アフリカツメガエルの心臓の形態形成におけるActomyosinの役割 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | The Role of Actomyosin Contraction Force in Heart Morphogenesis of Xenopus Tadpole Larvae | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | heart morphogenesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | actomyosin contraction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tadpole larva | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Xenopus laevis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | heart morphogenesis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | actomyosin contraction | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | tadpole larva | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Xenopus laevis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
秋永, 薫
× 秋永, 薫× 茂木, 和枝× 安積, 良隆× 豊泉, 龍児 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Many researchers have long been investigating the mechanism of actomyosin contraction. Actomyosin is an interesting macromolecular complex, which enables energy conversion that generates mechanical force very efficiently at a temperature compatible for animals. Vertebrate embryos gradually develop striated muscles, and the embryos can make various body movements by muscular contraction as early as at the late embryonic stage before hatching. In lower vertebrates, pulsation of the heart is also observable in late stage embryos. Here, we examined whether active contraction of actomyosin is necessary for normal morphogenesis of the embryonic/larval heart. Xenopus early larvae are very transparent, so they are suitable to observe the heart morphology and its contractile activities non-invasively. From such a viewpoint, the Xenopus larva is an ideal model organism. Thus, we first administered Blebbistatin, an inhibitor of actomyosin contraction, to Xenopus late stage embryos, and reared them until the early larval stage. As the results, hypoplasia of heart morphogenesis was frequently induced. As a next step, we intended to perform a comparable experiment; we administered Omecamtiv, a cardiotonic reagent, to potentiate the actomyosin contraction of the developing heart. Unexpectedly, Omecamtiv also induced a dwarf heart at a high frequency. Immunostaining using the antibody for muscles revealed that both Blebbistatin and Omecamtiv caused changes of fine structures of the heart. Based on these complementary experiments, we concluded that moderate contraction of actomyosin is essential for the normal asymmetric morphogenesis of the heart. Finally we report that Phenylhydrazine significantly induced hyperplasia of the heart, and fasciculation of the ventricular muscles was disordered after Phenylhydrazine treatment. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 原著 | |||||
| 書誌情報 |
en : Science Journal of Kanagawa University 巻 33, p. 67-79, 発行日 2022-07-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学総合理学研究所 | |||||
| 出版者 | ||||||
| 出版者 | Research Institute for Integrated Science, Kanagawa University | |||||
