WEKO3
アイテム
腊葉標本DNAのMIG-seq法による利用可能性・解析手法の検討
http://hdl.handle.net/10487/00016004
http://hdl.handle.net/10487/000160041ee355f6-4c4e-494d-b258-ae65ad1ca7f8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
14 腊葉標本DNAのMIG-seq法による利用可能性・解析手法の検討 (1.3 MB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-18 | |||||
| タイトル | ||||||
| タイトル | 腊葉標本DNAのMIG-seq法による利用可能性・解析手法の検討 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Examination of Possibilities and Methods for MIG-seq Analysis Using Plant Herbarium Specimen-Derived DNA | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Plant herbarium specimen | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MIG-seq | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | next generation sequencer | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | stacks | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Plant herbarium specimen | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | MIG-seq | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | next generation sequencer | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | stacks | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
岩崎, 貴也
× 岩崎, 貴也× 小玉, あすか× 松尾, 歩× 陶山, 佳久× 大西, 亘× 尾関, 雅章× 中濱, 直之× 山本, 薫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Herbarium specimens hold their DNA information at the time of collection from fields. This DNA information provides us with the opportunity to directly compare the past and present. However, it is difficult to analyze specimen DNA in the usual way because the DNA has been fragmented due to the effects of ultraviolet light and chemical fumigation. Therefore, in this study, we examined possibilities and methods of MIG-seq analysis using specimens of six plant species. As the results, several thousand or more loci were sequenced on average in all species, indicating that MIG-seq analysis is also useful for plant specimen-derived DNA. The number of loci and number of sequence reads obtained by the next-generation sequencer were significantly positively correlated, and the number of loci continues to increase without convergence even if the number of leads exceeds 300,000. Although the number of loci after correction of the number of reads decreased in old specimen samples of three species, a relatively large number of loci could be obtained in many samples in the case of specimens prepared around 1980. Also a sufficient number of loci were also sequenced in some samples of older specimens. Therefore, as long as the specimen condition is good, MIG-seq analysis should be useful even for a fairly old sample. Moreover, when the sequence data from multiple independent library constructions were integrated, the number of loci continued to rise even when the number of reads exceeded about 800,000. In conclusion, the most effective way to perform MIG-seq analysis for plant specimen-derived DNA would be to carry out multiple independent library preparations and obtain as many reads as possible. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 原著 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2018年度神奈川大学総合理学研究所共同研究助成論文 | |||||
| 書誌情報 |
en : Science Journal of Kanagawa University 巻 30, p. 89-96, 発行日 2019-06-30 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他の言語のタイトル | ||||||
| その他のタイトル | Examination of Possibilities and Methods for MIG-seq Analysis Using Plant Herbarium Specimen-Derived DNA | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学総合理学研究所 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Departmental Bulletin Paper | |||||
