WEKO3
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Benzothiazolylphenol–Substituted Ketoester is a Useful Fluorescent Probe for Detection of the Mitochondrion in Sea Urchin Sperm
http://hdl.handle.net/10487/5273
http://hdl.handle.net/10487/5273702f727a-13ad-44ef-b510-3be2a2aed747
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
05 19 19-26.pdf (3.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-12-21 | |||||
| タイトル | ||||||
| タイトル | Benzothiazolylphenol–Substituted Ketoester is a Useful Fluorescent Probe for Detection of the Mitochondrion in Sea Urchin Sperm | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | sea urchin sperm | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ketoester | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dioxetane | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mitochondrion | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fluorescence microscopy | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Kazama, Makoto
× Kazama, Makoto× Kanda, Keiichiro× Tanimura, Masatoshi× Hino, Akiya× 日野, 晶也× Matsumoto, Masakatsu× 松本, 正勝× Suzuki, Suechika× 鈴木, 季直 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | One of the ketoesters derived from benzothiazolylphenol-substituted dioxetane, benzothiazolylphenol-substituted ketoester (TPKE), demonstrates fluorescence in a 0.1 M NaOH 1). In this study, the fluorescent staining of a living cell with TPKE was demonstrated by fluorescence microscopy. When sperm from two species of sea urchins—Pseudocentrotus depressus and Anthocidaris crassispina—were used as biological materials, TPKE showed a fluorescent signal in the midpiece that was composed of a single mitochondrion. The ratio of fluorescent signal intensity to background noise (S/N) was high in the sperm stained with 1.0–5.0 μg/ml TPKE in normal artificial seawater (pH 8.0). The S/N ratio decreased in acidic seawater (pH 6.0); acidic conditions repress respiratory activity in sea urchin sperm. Moreover, in the presence of the respiratory chain inhibitor antimycin A and the uncoupler carbonyl cyanide p--trifluoromethoxyphenyl-hydrazone, the sperm showed faint or no fluorescence in normal artificial seawater (pH 8.0). Sea urchin sperm stained with TPKE after fixation showed faint or no fluorescence. These results suggest that TPKE is a potential fluorescent probe of living sea urchin sperm mitochondria with high respiratory activities. |
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| 書誌情報 |
Science Journal of Kanagawa University 巻 19, p. 19-26, 発行日 2008-06-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
