{"created":"2023-05-15T11:46:25.333503+00:00","id":6959,"links":{},"metadata":{"_buckets":{"deposit":"8055746e-06d6-4bd5-a3d8-dc9bed1b4ae0"},"_deposit":{"created_by":1,"id":"6959","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6959"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006959","sets":["376:377:378:558"]},"author_link":["20930","20929","20926","20933","20928","20927","20925","20931","20932"],"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"26","bibliographicPageStart":"19","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"One of the ketoesters derived from benzothiazolylphenol-substituted dioxetane,\nbenzothiazolylphenol-substituted ketoester (TPKE), demonstrates fluorescence in a 0.1 M\nNaOH 1). In this study, the fluorescent staining of a living cell with TPKE was demonstrated\nby fluorescence microscopy. When sperm from two species of sea urchins—Pseudocentrotus\ndepressus and Anthocidaris crassispina—were used as biological materials, TPKE showed a\nfluorescent signal in the midpiece that was composed of a single mitochondrion. The ratio\nof fluorescent signal intensity to background noise (S/N) was high in the sperm stained with\n1.0–5.0 μg/ml TPKE in normal artificial seawater (pH 8.0). The S/N ratio decreased in\nacidic seawater (pH 6.0); acidic conditions repress respiratory activity in sea urchin sperm.\nMoreover, in the presence of the respiratory chain inhibitor antimycin A and the uncoupler\ncarbonyl cyanide p--trifluoromethoxyphenyl-hydrazone, the sperm showed faint or no\nfluorescence in normal artificial seawater (pH 8.0). Sea urchin sperm stained with TPKE\nafter fixation showed faint or no fluorescence. These results suggest that TPKE is a\npotential fluorescent probe of living sea urchin sperm mitochondria with high respiratory\nactivities.","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kazama, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Keiichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanimura, Masatoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hino, Akiya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日野, 晶也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Masakatsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本, 正勝"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Suechika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 季直"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"05 19 19-26.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"05 19 19-26.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6959/files/05 19 19-26.pdf"},"version_id":"25b48835-ef8d-4029-918f-f34dd843414e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"sea urchin sperm","subitem_subject_scheme":"Other"},{"subitem_subject":"ketoester","subitem_subject_scheme":"Other"},{"subitem_subject":"dioxetane","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondrion","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence microscopy","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Benzothiazolylphenol–Substituted Ketoester is a Useful Fluorescent Probe for Detection of the Mitochondrion in Sea Urchin Sperm","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Benzothiazolylphenol–Substituted Ketoester is a Useful Fluorescent Probe for Detection of the Mitochondrion in Sea Urchin Sperm"}]},"item_type_id":"3","owner":"1","path":["558"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-21"},"publish_date":"2009-12-21","publish_status":"0","recid":"6959","relation_version_is_last":true,"title":["Benzothiazolylphenol–Substituted Ketoester is a Useful Fluorescent Probe for Detection of the Mitochondrion in Sea Urchin Sperm"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:44:43.448496+00:00"}