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Cloning and Sequencing of the Poly (3-hydroxybutyrate)(PHB) Synthase Gene from Purple Non-Sulfur Bacteria Rhodospirillum centenum and Expression of the Gene in Escherichia coli
http://hdl.handle.net/10487/1448
http://hdl.handle.net/10487/14488ce57d4f-20bb-4faa-8157-99709ae93278
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kana-2-2-0003.pdf (1.8 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-23 | |||||
| タイトル | ||||||
| タイトル | Cloning and Sequencing of the Poly (3-hydroxybutyrate)(PHB) Synthase Gene from Purple Non-Sulfur Bacteria Rhodospirillum centenum and Expression of the Gene in Escherichia coli | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | poly (3-hydroxybutyrate) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PHB | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PHB synthase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | purple non-sulfur bacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Rhodospirillum centenum | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phbC | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Shiraki, Mari
× Shiraki, Mari× 白木, 麻里× Nagano, Takashi× Suzuki, Emiko× Saito, Terumi× 齊藤, 光實 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A 10-kb genomic fragment was isolated from Rhodospirillum centenum by Southern-hybridization and colony-hybridization, using a probe amplified by PCR with oligo-nucleotide primers constructed from a sequence conserved in poly (3-hydro xybutyrate)(PHB) synthase genes. After subcloning of an approximately 3-kb fragment (SmaI-EcoRV) that caused the production of PHB in Escherichia coli in the presence of β-ketothiolase (phbA), an acetoacety-CoA reductase (phbB) gene from Ralstonia eutropha H16 was obtained and sequenced. This fragment contained an open reading frame (ORF) whose amino acid sequence was highly similar to the sequences of other known PHB synthase genes, especially to a synthase from Azospirillum brasilense (74% identity). | |||||
| 書誌情報 |
Science Journal of Kanagawa University 巻 17, p. 13-19, 発行日 2006-05-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学 | |||||
