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器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究
http://hdl.handle.net/10487/00018154
http://hdl.handle.net/10487/00018154c9ced248-3923-400a-ad70-01666c74da0e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
08 器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究 (4.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-09-20 | |||||
| タイトル | ||||||
| タイトル | 器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | In Vitro Approach to Decode the Mechanisms of Coiling Morphogenesis of Xenopus Larval Gut | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | intestinal smooth muscle | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | myosin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | actin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Wnt signaling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | organ culture | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | intestinal smooth muscle | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | myosin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | actin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Wnt signaling | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | organ culture | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
秋永, 薫
× 秋永, 薫× 安積, 良隆× 茂木, 和枝× 豊泉, 龍児 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Xenopus laevis is a good model organism for the study of gut morphogenesis during larval development. Because its larva has a very translucent abdominal epidermis, we can make noninvasive observations consistently during gut coiling morphogenesis for a long time. Recently, we developed an in vitro organ culture system that can recapitulate the early gut looping morphogenesis of Xenopus larvae. With a combination of in vivo and in vitro experiments, we have tried to elucidate the mechanisms of Xenopus larval gut coiling morphogenesis. In this study, we first observed the distribution of phosphorylated myosin (p-myosin) in the larval gut. Myosin-positive filamentous structures, which might correspond to gut smooth muscle cells, aligned in the transverse direction on the gut surface in the duodenum. Next, the cellular meshwork of the p-myosin-positive cells was organized by their circular and longitudinal orientations. Anisotropic twisting of the meshwork was observed at the center of the coiled gut. A pharmacological approach using a gut organ culture system suggested that contraction by actomyosin interaction is essential for gut coiling morphogenesis. Administration of inhibitors for myosin II ATPase and Rho-kinase significantly perturbed gut looping in vitro . Finally, we examined whether cell-to-cell communication by Wnt signaling is involved in the gut coiling. Administration of Wnt inhibitors also prevented successful gut looping. Taken together, we propose that reciprocal contraction by peristaltic movement of the nascent gut smooth muscle cells is needed for proper gut coiling morphogenesis in anuran amphibians. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 原著 | |||||
| 書誌情報 |
en : Science Journal of Kanagawa University 巻 33, p. 57-65, 発行日 2022-07-30 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-0483 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12068302 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 神奈川大学総合理学研究所 | |||||
| 出版者 | ||||||
| 出版者 | Research Institute for Integrated Science, Kanagawa University | |||||
