{"created":"2023-05-15T11:52:42.655790+00:00","id":15089,"links":{},"metadata":{"_buckets":{"deposit":"3e82b48c-d92a-4ac4-8f98-ab72e33de071"},"_deposit":{"created_by":10,"id":"15089","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"15089"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00015089","sets":["376:377:378:1428"]},"author_link":["37179","34086","37180","34316","37177","34086","37178","34316"],"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-07-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"65","bibliographicPageStart":"57","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Xenopus laevis is a good model organism for the study of gut morphogenesis during larval development. Because its larva has a very translucent abdominal epidermis, we can make noninvasive observations consistently during gut coiling morphogenesis for a long time. Recently, we developed an in vitro organ culture system that can recapitulate the early gut looping morphogenesis of Xenopus larvae. With a combination of in vivo and in vitro experiments, we have tried to elucidate the mechanisms of Xenopus larval gut coiling morphogenesis. In this study, we first observed the distribution of phosphorylated myosin (p-myosin) in the larval gut. Myosin-positive filamentous structures, which might correspond to gut smooth muscle cells, aligned in the transverse direction on the gut surface in the duodenum. Next, the cellular meshwork of the p-myosin-positive cells was organized by their circular and longitudinal orientations. Anisotropic twisting of the meshwork was observed at the center of the coiled gut. A pharmacological approach using a gut organ culture system suggested that contraction by actomyosin interaction is essential for gut coiling morphogenesis. Administration of inhibitors for myosin II ATPase and Rho-kinase significantly perturbed gut looping in vitro . Finally, we examined whether cell-to-cell communication by Wnt signaling is involved in the gut coiling. Administration of Wnt inhibitors also prevented successful gut looping. Taken together, we propose that reciprocal contraction by peristaltic movement of the nascent gut smooth muscle cells is needed for proper gut coiling morphogenesis in anuran amphibians.","subitem_description_type":"Abstract"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学総合理学研究所"},{"subitem_publisher":"Research Institute for Integrated Science, Kanagawa University"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"秋永, 薫"}],"nameIdentifiers":[{"nameIdentifier":"37177","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安積, 良隆"}],"nameIdentifiers":[{"nameIdentifier":"34086","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"34086","nameIdentifierScheme":"ローカル著者ID","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"茂木, 和枝"}],"nameIdentifiers":[{"nameIdentifier":"37178","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"豊泉, 龍児"}],"nameIdentifiers":[{"nameIdentifier":"34316","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"34316","nameIdentifierScheme":"ローカル著者ID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-20"}],"displaytype":"detail","filename":"08 器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究.pdf","filesize":[{"value":"4.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"08 器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究","url":"https://kanagawa-u.repo.nii.ac.jp/record/15089/files/08 器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究.pdf"},"version_id":"148ca72c-a8cc-4001-855a-fa643c2236ef"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"intestinal smooth muscle","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin","subitem_subject_scheme":"Other"},{"subitem_subject":"actin","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt signaling","subitem_subject_scheme":"Other"},{"subitem_subject":"organ culture","subitem_subject_scheme":"Other"},{"subitem_subject":"intestinal smooth muscle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"actin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt signaling","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"organ culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究","subitem_title_language":"ja"},{"subitem_title":"In Vitro Approach to Decode the Mechanisms of Coiling Morphogenesis of Xenopus Larval Gut","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["1428"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-20"},"publish_date":"2022-09-20","publish_status":"0","recid":"15089","relation_version_is_last":true,"title":["器官培養系を用いたアフリカツメガエル幼生腸管の形態形成の研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-06-19T02:11:43.777673+00:00"}