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コルヒチンを用いたシロイヌナズナ(Arabidopsis thaliana)4倍体及び8倍体の効率的な作出方法
http://hdl.handle.net/10487/00017136
http://hdl.handle.net/10487/00017136c727f346-7afc-4d4d-b6f9-6be7b551efad
名前 / ファイル | ライセンス | アクション |
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13 コルヒチンを用いたシロイヌナズナ(Arabidopsis thaliana)4倍体及び8倍体の効率的な作出方法 (2.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2021-08-18 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | コルヒチンを用いたシロイヌナズナ(Arabidopsis thaliana)4倍体及び8倍体の効率的な作出方法 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | An Efficient Method of Tetraploid and Octaploid Induction in Arabidopsis thaliana Using Colchicine Treatment | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arabidopsis thaliana | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tetraploid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | octaploid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | colchicine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | flow cytometry | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Arabidopsis thaliana | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | tetraploid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | octaploid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | colchicine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | flow cytometry | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
菊池, 涼夏
× 菊池, 涼夏× 岩元, 明敏 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Colchicine gel treatment is one of the major methods used to induce polyploid strains in plants. In this study, we examined several conditions of colchicine gel treatment in the diploid and tetraploid of Arabidopsis thaliana (ecotype: Columbia) to establish the most efficient method of tetraploid and octaploid induction, and compared the induction efficiency with that of one-drop colchicine solution treatment, which was previously reported. The shoot apex of diploid and tetraploid seedlings was treated with colchicine gel (0.5% or 1.0% colchicine dissolved in 1.0% agarose) for several days. Thus, 0.5% colchicine gel treatment of the diploid for two days induced tetraploid (7.5% of treated seedlings) most efficiently under all examined conditions, suggesting that one-drop colchicine solution treatment of the diploid is more effi-cient than colchicine gel treatment. 0.5% colchicine gel treatment of the tetraploid for two days induced ctaploid (5.0% of treated seedlings) most efficiently, which is similar to the one-drop colchicine solution treatment of the diploid. The survival rate of colchicine gel-treated seed-lings was much lower than that of one-drop colchicine-solution-treated seedlings. The survival rate difference resulted in a lower efficiency of polyploid induction by colchicine gel treatment. These results suggest that one-drop colchicine solution treatment of the tetraploid can most ef-ficiently induce octaploid. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | テクニカルノート | |||||
書誌情報 |
en : Science Journal of Kanagawa University 巻 32, p. 81-86, 発行日 2021-06-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1880-0483 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12068302 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他の言語のタイトル | ||||||
その他のタイトル | An Efficient Method of Tetraploid and Octaploid Induction in Arabidopsis thaliana Using Colchicine Treatment | |||||
出版者 | ||||||
出版者 | 神奈川大学総合理学研究所 | |||||
出版者 | ||||||
出版者 | Research Institute for Integrated Science, Kanagawa University | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Departmental Bulletin Paper |