WEKO3
アイテム
{"_buckets": {"deposit": "324aac4a-e62e-4759-a159-e05352d3722e"}, "_deposit": {"created_by": 1, "id": "6969", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "6969"}, "status": "published"}, "_oai": {"id": "oai:kanagawa-u.repo.nii.ac.jp:00006969", "sets": ["558"]}, "author_link": ["21002", "21006", "21009", "21005", "21008", "21003", "21007", "21004"], "item_3_alternative_title_20": {"attribute_name": "その他の言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Examination of the Most Suitable Preparation Method for Pollen Observation by Scanning Electron Microscope"}]}, "item_3_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-06-30", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "86", "bibliographicPageStart": "81", "bibliographicVolumeNumber": "19", "bibliographic_titles": [{"bibliographic_title": "Science Journal of Kanagawa University"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To examine the most suitable method to prepare pollen for scanning electron\nmicroscope observation, several fixation methods and dehydration (or drying) methods were\napplied to Arabidopsis and lily pollen obtained from flowers before and after flowering.\nLight and confocal laser microscope observations revealed that, in both plants, pollen\nobtained before flowering was swollen and wet, while that obtained after flowering was\nshrunken and dry. For scanning electron microscopy, pollen was unfixed or fixed with 50%\n(eventually 100%) acetone, FAA (5% formalin with 50% ethanol and 5% acetic acid) or 6%\nglutaraldehyde solutions. It was then dried naturally in air or artificially by either critical\npoint-dry or freeze-dry machines, respectively. The results indicated that, for scanning\nelectron microscope observation of dry pollen, the most suitable treatment is natural drying\nwithout fixation. On the other hand, to observe wet pollen, the combination of FAA or\nglutaraldehyde fixation with artificial drying using either machines is preferable, and\nfixation with acetone is unsuitable.", "subitem_description_type": "Abstract"}]}, "item_3_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "テクニカルノート", "subitem_description_type": "Other"}]}, "item_3_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "神奈川大学"}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12068302", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1880-0483", "subitem_source_identifier_type": "ISSN"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "重田, 英子"}], "nameIdentifiers": [{"nameIdentifier": "21002", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shigeta, Eiko"}], "nameIdentifiers": [{"nameIdentifier": "21003", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩元, 明敏"}], "nameIdentifiers": [{"nameIdentifier": "21004", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwamoto, Akitoshi"}], "nameIdentifiers": [{"nameIdentifier": "21005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "風間, 真"}], "nameIdentifiers": [{"nameIdentifier": "21006", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kazama, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "21007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木, 季直"}], "nameIdentifiers": [{"nameIdentifier": "21008", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Suechika"}], "nameIdentifiers": [{"nameIdentifier": "21009", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-11-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "15 19 81-86.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "15 19 81-86.pdf", "url": "https://kanagawa-u.repo.nii.ac.jp/record/6969/files/15 19 81-86.pdf"}, "version_id": "ffad88d8-a124-4368-8b13-ae66a61f3bbf"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "pollen", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Arabidopsis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lily", "subitem_subject_scheme": "Other"}, {"subitem_subject": "preparation methods", "subitem_subject_scheme": "Other"}, {"subitem_subject": "scanning electron microscopyrs_j", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "走査型電子顕微鏡による花粉観察のための最適処理法の検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "走査型電子顕微鏡による花粉観察のための最適処理法の検討"}]}, "item_type_id": "3", "owner": "1", "path": ["558"], "permalink_uri": "http://hdl.handle.net/10487/5280", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-12-21"}, "publish_date": "2009-12-21", "publish_status": "0", "recid": "6969", "relation": {}, "relation_version_is_last": true, "title": ["走査型電子顕微鏡による花粉観察のための最適処理法の検討"], "weko_shared_id": -1}
走査型電子顕微鏡による花粉観察のための最適処理法の検討
http://hdl.handle.net/10487/5280
http://hdl.handle.net/10487/52803b31cd41-2398-42c9-abcc-43a6c543611a
名前 / ファイル | ライセンス | アクション |
---|---|---|
15 19 81-86.pdf (2.1 MB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2009-12-21 | |||||
タイトル | ||||||
タイトル | 走査型電子顕微鏡による花粉観察のための最適処理法の検討 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pollen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arabidopsis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lily | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | preparation methods | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | scanning electron microscopyrs_j | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
重田, 英子
× 重田, 英子× Shigeta, Eiko× 岩元, 明敏× Iwamoto, Akitoshi× 風間, 真× Kazama, Makoto× 鈴木, 季直× Suzuki, Suechika |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To examine the most suitable method to prepare pollen for scanning electron microscope observation, several fixation methods and dehydration (or drying) methods were applied to Arabidopsis and lily pollen obtained from flowers before and after flowering. Light and confocal laser microscope observations revealed that, in both plants, pollen obtained before flowering was swollen and wet, while that obtained after flowering was shrunken and dry. For scanning electron microscopy, pollen was unfixed or fixed with 50% (eventually 100%) acetone, FAA (5% formalin with 50% ethanol and 5% acetic acid) or 6% glutaraldehyde solutions. It was then dried naturally in air or artificially by either critical point-dry or freeze-dry machines, respectively. The results indicated that, for scanning electron microscope observation of dry pollen, the most suitable treatment is natural drying without fixation. On the other hand, to observe wet pollen, the combination of FAA or glutaraldehyde fixation with artificial drying using either machines is preferable, and fixation with acetone is unsuitable. |
|||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | テクニカルノート | |||||
書誌情報 |
Science Journal of Kanagawa University 巻 19, p. 81-86, 発行日 2008-06-30 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1880-0483 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12068302 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他の言語のタイトル | ||||||
その他のタイトル | Examination of the Most Suitable Preparation Method for Pollen Observation by Scanning Electron Microscope | |||||
出版者 | ||||||
出版者 | 神奈川大学 |