In the winter of 2017, algal communities in the Sagami Bay were analyzed. In the mouth of the estuary of the Sagami River, algal communities different from those in offshorewater bodies were found. The structures of the 2 different communities in the mouth and offshore water-bodies were also analyzed by Amplicon analysis with NGS. DNA samples required for the analysis were obtained by extraction and purification from cells collected by the filtration of seawater. Although a sufficient number of OTU could be determined, they showed completely different phylogenic relationships from the results of microscopic observations. The index region of psbA in this study was not suitable for DNA barcoding.
内容記述
原著
2017年度神奈川大学総合理学研究所共同研究助成論文
雑誌名
Science Journal of Kanagawa University
巻
29
ページ
89 - 92
発行年
2018-06-30
ISSN
1880-0483
書誌レコードID
AA12068302
著者版フラグ
publisher
その他の言語のタイトル
Trial Application of Next-Generation DNA Sequencer for the Estimation of Structures of Natural Algal Communities in the Estuary of the Sagami River
相模川河口域天然藻類群集の群集構造の推定への次世代シークエンサーの試験的適用
15 相模川河口域天然藻類群集の群集構造の推定への次世代シークエンサーの試験的適用
(1.26MB)
