{"created":"2023-05-15T11:46:50.286801+00:00","id":7509,"links":{},"metadata":{"_buckets":{"deposit":"0960aab0-2905-4270-928b-3431cff49d82"},"_deposit":{"created_by":1,"id":"7509","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"7509"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00007509","sets":["376:377:574:584"]},"author_link":["23933","23934","23935","23938","23936","23939","23941","23937","23940","23932"],"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"140","bibliographicPageStart":"133","bibliographicVolumeNumber":"2003","bibliographic_titles":[{"bibliographic_title":"年報"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"将来的にはタマネギで高発現するアリイナーゼ遺伝子を利用して、タマネギ内で生分解性プラスチック(Bio-Degradable Plastic;BDP)合成に必要な遺伝子を高発現させ、タマネギにBDPを作らせる計画である。本年度はアリイナーゼ遺伝子のプロモーター及びカリフラワーモザイクウィルス35SプロモーターとアリイナーゼcDNAとレポーター遺伝子であるGFPを新しく開発された遺伝子組み換え技術を利用して連結し、融合遺伝子35S::AlliC::GFPとAlliP::AlliC::GFPを作製した。これを遺伝子銃を用いて、タマネギの鱗茎葉の表皮細胞に導入して、GFP遺伝子の発現レベルを調べ、プロモーターの遺伝子発現能力を調べた。また現在、タマネギ形質転換体での外来遺伝子発現研究のパイロット実験として35S::GFP::GUS融合遺伝子をタマネギ細胞に導入して形質転換体を作製して、その成長過程を調べている。","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11822302","subitem_source_identifier_type":"NCID"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安積, 良隆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Azumi, Yoshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"齋藤, 光実"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Terumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本, 佳代"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"北, 宜裕"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kita, Nobuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上西, 愛子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野村, 研"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nomura, K."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"kana-1-10-0009.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kana-1-10-0009.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/7509/files/kana-1-10-0009.pdf"},"version_id":"a9515524-7565-4278-8f07-82c08d0c0789"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"遺伝子組換えタマネギに生分解性プラスチックを生合成させる試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子組換えタマネギに生分解性プラスチックを生合成させる試み"}]},"item_type_id":"3","owner":"1","path":["584"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-23"},"publish_date":"2009-02-23","publish_status":"0","recid":"7509","relation_version_is_last":true,"title":["遺伝子組換えタマネギに生分解性プラスチックを生合成させる試み"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T08:33:21.304545+00:00"}