@article{oai:kanagawa-u.repo.nii.ac.jp:00007294,
 author = {釜野, 徳明 and Kamano, Yoshiaki and 五味, 和枝 and Gomi, Kazue and 張, 恵平 and Zhang, Hui-ping and 小竹, 文乃 and Kotake, Ayano and 日野, 晶也 and Hino, Akiya and 木津, 治久 and Kizu, Haruhisa and 西川, 輝昭 and Nishikawa, Teruaki and 薗部, 徹 and Sonobe, Thoru and 大村, 武雄 and Ohmura, Takeo},
 journal = {年報},
 month = {Mar},
 note = {Two immunosuppressive sterols, cholesta-5,23E-diene-3β, 25-diol (trans-liagosterol) (A) and cholesta-5,25 (26)-diene-3β, 24ζ-diol (B) were isolated respectively from the Sagami Gulf Hydrozoa Tubularia sp. by the guide of Mitogen bioassey. Structural determination was accomplished mainly by utilizing high-field (400MHz) 2D-NMR experiments, comparing to the spectra of cholesterol. Although these sterols, A and B, were known as the minor components of marine red alga, the presence in marine animal Hydrozoa such as Tubularia sp. was a new discovery. Sterol A inhibited T lymphocyte proliferation which was induced by plant lectins, concanavalin A (Con-A) and phytochemagglutinin (PHA), more than B lymphocyte proliferation which was induced by lipopolysaccharide (LPS). The activity of sterol B was more week than that of sterol A. On the other hand, by the Mitogen bioassay with hydrocortisone (HC) as a standard inhibitor and related five steroids, β-sitosterol (C), stigmesterol acetate (D), resibufogenin (E), 14,21-epoxy-3 β-hydroxy-5 β-chola-20 (21), 22-dienoic acid (F) and ergosterol (G), the inhibition of sterol A for PHA response was found to be most strong in them. However, for LPS response, the inhibition of hydrocortisone (HC) was more powerful than that of sterol A. Also, the immunosuppresive activity of related steroids, C, D, E and G was little found.},
 pages = {11--20},
 title = {相模湾のクダウミヒドラ Tubularia sp. から単離した免疫抑制活性ステロールの構造と活性},
 volume = {'95},
 year = {1996}
}