{"created":"2023-05-15T11:46:23.763019+00:00","id":6924,"links":{},"metadata":{"_buckets":{"deposit":"bc04f44d-decd-48ab-93a8-c1eaa0bdd7cb"},"_deposit":{"created_by":1,"id":"6924","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6924"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006924","sets":["376:377:378:556"]},"author_link":["20701","20700","20706","20704","20710","20705","20702","20708","20707","20709","20703","20699"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Visualization of the Semitranslucent Brain Ventricle and Its Fluid Flow Using Microinjection Technique for Albino Xenopus laevis Larvae"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-05-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"61","bibliographicPageStart":"53","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In vertebrates, the central nervous system (CNS) develops as a tube called the neural tube. Ependymal cells seal the inner surface of the brain ventricle, and movement of the cilia on the apical surface of the ependymal cells generates fluid flow called cerebrospinal fluid flow. The role of cerebrospinal fluid flow for the process of neurogenesis and regionalization of the CNS remains unveiled. In this study, using albino larvae of Xenopus laevis, we report a new methodology to clearly visualize the semitranslucent morphology of the brain ventricle and patterning of the fluid flow within the cavity during amphibian CNS development. Microinjection of the quantum dot (fluorescent nanocrystal) through the roof plate of the fourth ventricle rapidly and efficiently visualized the whole brain ventricle under fluorescent micrography, enabling us to trace the complicated morphology during development of the third, fourth and lateral ventricles. Microinjection of polystyrene beads (3.1μm in diameter) into the fourth ventricle also efficiently dispersed into every corner of the brain ventricle. This technique revealed that fluid flow within fourth ventricle displays dorso-ventral asymmetry. In 60% of the embryos examined, the rearward fluid flow within the third ventricle shifted to the left at the dorsal portion of the ventricle, whereas, in the other larvae, it was quite bilateral. These results suggest that fluid flow within the developing CNS is generated by a highly integrated, position-dependent metachronal wave of cilia on ependymal cell surfaces. This report is the first description of left-right asymmetric fluid flow in the brain ventricle of vertebrates, encouraging us to examine the relationships between the laterality of tadpole behavior and left-right asymmetry underlying the molecular anatomy of the developing brain.","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松谷, 武嗣"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"茂木, 和枝"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日野, 晶也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小笠原, 強"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹内, 重夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"豊泉, 龍児"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuya, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mogi, Kazue"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hino, Akiya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogasawara, Tsuyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeuchi, Shigeo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toyoizumi, Ryuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"SJKU Vol 17 53-61.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SJKU Vol 17 53-61.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6924/files/SJKU Vol 17 53-61.pdf"},"version_id":"e3f0ba11-7fb6-4fb9-8a4f-e9b63ba86ba4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"albino","subitem_subject_scheme":"Other"},{"subitem_subject":"cerebrospinal fluid flow","subitem_subject_scheme":"Other"},{"subitem_subject":"nanocrystal","subitem_subject_scheme":"Other"},{"subitem_subject":"polystyrene beads","subitem_subject_scheme":"Other"},{"subitem_subject":"left-right asymmetry","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アルビノ-アフリカツメガエル胚に対する微量注射技術を用いた半透明な脳室形態並びに脳室内液流の可視化(2005年度神奈川大学総合理学研究所協同研究助成論文)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アルビノ-アフリカツメガエル胚に対する微量注射技術を用いた半透明な脳室形態並びに脳室内液流の可視化(2005年度神奈川大学総合理学研究所協同研究助成論文)"}]},"item_type_id":"3","owner":"1","path":["556"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-23"},"publish_date":"2009-02-23","publish_status":"0","recid":"6924","relation_version_is_last":true,"title":["アルビノ-アフリカツメガエル胚に対する微量注射技術を用いた半透明な脳室形態並びに脳室内液流の可視化(2005年度神奈川大学総合理学研究所協同研究助成論文)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:45:24.036612+00:00"}