{"created":"2023-05-15T11:46:22.863453+00:00","id":6904,"links":{},"metadata":{"_buckets":{"deposit":"f866b675-28fa-453e-b369-12fcdce80ac5"},"_deposit":{"created_by":1,"id":"6904","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6904"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006904","sets":["376:377:378:555"]},"author_link":["20564","20566","20560","20561","20563","20565","20562","20567"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Novel intracellular 3-hydroxybutyrate-oligomer hydrolase in Ralstonia eutropha H16"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-05-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"71","bibliographicPageStart":"67","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ralstonia eutropha H16 mobilizes intracellularly accumulated poly(3-hydroxybutyrate) with intracellular poly(3-hydroxybutyrate) depolymerases. Different types of depolymerase, PhaZ1 and its homologs, account for the mobilization significantly but incompletely, and it is proposed that further unknown depolymerases or related enzymes complement the mobilization. A novel hydrolase was therefore investigated. The amino acid sequence of the intracellular 3HB-oligomer hydrolase of Acidovoraxsp. SA1 and the genome sequence of Ralstonia solanacearum, a close relative of R. eutropha, revealed a candidate for the hydrolase gene. The DNA sequence of its hydrolase was used for the design of a pair of primes. With chromosomal DNA of R. eutropha as a template, these primers were used to amplify about a 1.3-kbp fragment including the equivalent gene, phaZc. Then the phaZc gene was cloned by Southern hybridization using the amplified DNA fragment as a probe. The hydrolase (PhaZc) was purified from Escherichia coli overexpressing phaZc and characterized. Immunoblot analysis revealed that PhaZc exists mainly in the cytosolic fraction of R. eutropha. PhaZc degraded various 3-hydroxybutyrate-oligomers at a high rate and artificial amorphous poly (3-hydroxybutyrate) at a lower rate. A phaZ-rmLl mutation enhanced the deposition of PHB in the logarithmic phase in a nutrient-rich medium. PhaZc differs from the previously reported hydrolases in R. eutropha and is a novel type of intracellular 3HB-oligomer hydrolase, which participates in the mobilization of PHB along with other hydrolases.","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 照幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Teruyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"内野, 圭一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uchino, Keiichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安部, 智子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"齊藤, 光實"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Terumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"SJKU Vol 16 2005 67-71.pdf","filesize":[{"value":"564.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SJKU Vol 16 2005 67-71.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6904/files/SJKU Vol 16 2005 67-71.pdf"},"version_id":"ca7f6084-e30f-4822-b7d7-1fd144663dde"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ralstonia eutropha H16","subitem_subject_scheme":"Other"},{"subitem_subject":"poly(3-hydroxybutyrate)","subitem_subject_scheme":"Other"},{"subitem_subject":"PHB","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular 3HB-oligomer hydrolase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ralstonia eutropha H16の新規細胞内3-ビドロキシ酪酸オリゴマー分解酵素(2004年度神奈川大学総合理学研究所共同研究助成論文)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ralstonia eutropha H16の新規細胞内3-ビドロキシ酪酸オリゴマー分解酵素(2004年度神奈川大学総合理学研究所共同研究助成論文)"}]},"item_type_id":"3","owner":"1","path":["555"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-23"},"publish_date":"2009-02-23","publish_status":"0","recid":"6904","relation_version_is_last":true,"title":["Ralstonia eutropha H16の新規細胞内3-ビドロキシ酪酸オリゴマー分解酵素(2004年度神奈川大学総合理学研究所共同研究助成論文)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:45:49.329558+00:00"}