{"created":"2023-05-15T11:46:22.551151+00:00","id":6897,"links":{},"metadata":{"_buckets":{"deposit":"2b2b1f8b-d20f-44ec-ad94-2ef27ea1eb6f"},"_deposit":{"created_by":1,"id":"6897","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"6897"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00006897","sets":["376:377:378:555"]},"author_link":["20506","20504","20502","20507","20505","20509","20508","20511","20503","20510"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"The potentiation of Nodal signaling in the right lateral plate mesoderm inverts the left-right specification of the internal organs."}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-05-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"44","bibliographicPageStart":"35","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In Xenopus, multiple nodal-related genes are expressed during early embryogenesis. Among them, only Xenopus nodal related-1(Xnr-1) is expressed unilaterally in the left lateral plate mesoderm(LPM) at the late neurula-early tailbud stage. Early studies report that ectopic administration of Xnr-1 in the right hemisphere at the cleavage stage alters the left-right specification of the heart and visceral organs, or else makes a secondary axis. However, because Xnr-1 and other Xnrs function already at the blastula-gastrula stage, it is very difficult to evaluate the correct timing of the effects of excessively administered Xnr-1 from such a method. To elucidate the essential role of Xnr-1 within the left LPM, ectopic potentiation of Nodal signaling in the right lateral plate mesoderm was performed. Right-side injection of Nodal protein changed the laterality of Xnr-1 and Xenopuspitx2, but lefty, and fully (more than 90%) reversed the situs of the internal organs. Polyethyleneimine-based gene transfer of Xnr-1 mRNA in the right LPM also changed the laterality of pitx2 and fully (more than 90%) reversed the situs of the internal organs. Taken together, the potentiation of Xnr-1 signaling in the right LPM induces pitx2 in the right side and fully inverts the left-right axis of the heart and visceral organs, suggesting that the right LPM can transduce Nodal signaling, and only the absence of the Xnr-1 ligand silences the Nodal signaling in the right LPM. Normal left-right balance of Xnr-1 signaling is needed for the normal left-right specification of the internal organs.","subitem_description_type":"Abstract"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"豊泉, 龍児"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toyoizumi, Ryuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"茂木, 和枝"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mogi, Kazue"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日野, 晶也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hino, Akiya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小笠原, 強"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogasawara, Tsuyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹内, 重夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeuchi, Shigeo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"SJKU Vol 16 2005 35-44.pdf","filesize":[{"value":"759.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SJKU Vol 16 2005 35-44.pdf","url":"https://kanagawa-u.repo.nii.ac.jp/record/6897/files/SJKU Vol 16 2005 35-44.pdf"},"version_id":"56241508-7911-43df-9b3c-6b65cc6e92b2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Xenopus laevis","subitem_subject_scheme":"Other"},{"subitem_subject":"left-right asymmetry","subitem_subject_scheme":"Other"},{"subitem_subject":"neurula","subitem_subject_scheme":"Other"},{"subitem_subject":"Xnr-1","subitem_subject_scheme":"Other"},{"subitem_subject":"pitx2","subitem_subject_scheme":"Other"},{"subitem_subject":"microinjection","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アフリカツメガエル胚右側板中胚葉におけるNodalシグナリングの活性化は内臓左右軸を逆転する(2003-2004年度神奈川大学共同研究奨励助成論文)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アフリカツメガエル胚右側板中胚葉におけるNodalシグナリングの活性化は内臓左右軸を逆転する(2003-2004年度神奈川大学共同研究奨励助成論文)"}]},"item_type_id":"3","owner":"1","path":["555"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-23"},"publish_date":"2009-02-23","publish_status":"0","recid":"6897","relation_version_is_last":true,"title":["アフリカツメガエル胚右側板中胚葉におけるNodalシグナリングの活性化は内臓左右軸を逆転する(2003-2004年度神奈川大学共同研究奨励助成論文)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T15:45:58.653334+00:00"}