| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-13 |
| タイトル |
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|
タイトル |
Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning in Tetrahymena thermophila |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Akematsu, Takahiko
Loidl, Josef
Fukuda, Yasuhiro
Iwamoto, Masaaki
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
During sexual reproduction in the ciliate Tetrahymena thermophila, meiosis occurs in the germline micronucleus, resulting in the formation of four haploid micronuclei. Of these, only one is selected to evade autophagy, and subsequently migrates to the membrane junction with the partner cell for reciprocal pronuclear exchange. We previously demonstrated that the transmembrane protein Semi1 is essential for this nuclear migration. Semi1 is specifically expressed in mating cells and localizes to the periphery of the selected nucleus. Loss of Semi1 disrupts nuclear attachment to the junction, leading to infertility. However, the mechanism by which Semi1 positions the nucleus at the junction remains unclear. Here, we report that the Semi1-interacting protein, Semi2, is also necessary for proper nuclear positioning. Deletion of Semi2 results in the same nuclear mislocalization phenotype and infertility observed in Semi1 mutant cells. Semi2 colocalizes with Semi1, but in the absence of Semi1, Semi2 fails to exhibit perinuclear localization. The selected nucleus anchors to microtubules prior to migration, a process dependent on both Semi1 and Semi2. We propose a model in which Semi1 recruits Semi2 to the selected nucleus, facilitating the interaction between the nucleus and microtubules required for proper nuclear positioning at the membrane junction. |
|
言語 |
en |
| 書誌情報 |
en : Molecular Biology of the Cell
巻 36,
号 3,
p. 1-15,
発行日 2025-03-01
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| ISSN |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1939-4586 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1059-1524 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1091/mbc.E24-11-0503 |
| 権利 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
Creative Commons Attribution 4.0 international |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© 2025 Akematsu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
The American Society for Cell Biology |
|
言語 |
en |
Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning in Tetrahymena thermophila.pdf (12.1 MB)
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