{"created":"2023-05-15T11:51:03.913612+00:00","id":12910,"links":{},"metadata":{"_buckets":{"deposit":"5bbae720-2298-4768-855a-9f592fbe1ebf"},"_deposit":{"created_by":10,"id":"12910","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"12910"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00012910","sets":["376:377:378:1252"]},"author_link":["34086","34340","34086","34339"],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Preparation of Meiotic Chromosomes of Arabidopsis thaliana by Digest-and-Fix Method"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"72","bibliographicPageStart":"67","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Meiosis is an essential process for the production of gametes, and it consists of one round of chromosome replication and two rounds of mitosis. During meiosis, the chromosome number must be accurately halved, or subsequent generations produced by fertilization of gametes with irregular numbers of chromosomes will become aneuploids, which are lethal in most cases. This accuracy is guaranteed by homologous chromosome synapsis and even segregation. Therefore, synapsis and the following segregation are prerequisites for the production of normal gametes and the next generation. The molecular mechanism of chromosome control during meiosis of plants remains unclear. Unknown components such as ncRNA may be involved. In order to elucidate components of this mechanism, a new method of intact chromosome preparation is necessary. We explored the Digest-and-Fix method, which consists of two steps. First, digestion of pollen mother cells with cell wall-digesting enzymes while keeping their plasma membrane intact, and second, simultaneous fixing and spreading of chromosomes with Farmer’s solution onto slide glasses.","subitem_description_type":"Abstract"}]},"item_3_description_40":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Departmental Bulletin Paper","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"},{"subitem_description":"2018年度神奈川大学総合理学研究所共同研究助成論文","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学総合理学研究所"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安積, 良隆"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"笹本, 浜子"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-18"}],"displaytype":"detail","filename":"11 消化後固定法によるシロイヌナズナの減数分裂期染色体の調製.pdf","filesize":[{"value":"3.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"11 消化後固定法によるシロイヌナズナの減数分裂期染色体の調製","url":"https://kanagawa-u.repo.nii.ac.jp/record/12910/files/11 消化後固定法によるシロイヌナズナの減数分裂期染色体の調製.pdf"},"version_id":"b865794d-46d8-42fa-afe1-2e1056908463"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Arabidopsis thaliana","subitem_subject_scheme":"Other"},{"subitem_subject":"meiosis","subitem_subject_scheme":"Other"},{"subitem_subject":"pollen mother cell","subitem_subject_scheme":"Other"},{"subitem_subject":"chromosome","subitem_subject_scheme":"Other"},{"subitem_subject":"cell wall","subitem_subject_scheme":"Other"},{"subitem_subject":"Arabidopsis thaliana","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"meiosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pollen mother cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chromosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell wall","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"消化後固定法によるシロイヌナズナの減数分裂期染色体の調製","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"消化後固定法によるシロイヌナズナの減数分裂期染色体の調製","subitem_title_language":"ja"},{"subitem_title":"Preparation of Meiotic Chromosomes of Arabidopsis thaliana by Digest-and-Fix Method","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["1252"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-18"},"publish_date":"2020-03-18","publish_status":"0","recid":"12910","relation_version_is_last":true,"title":["消化後固定法によるシロイヌナズナの減数分裂期染色体の調製"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-06-19T02:05:35.563922+00:00"}