@article{oai:kanagawa-u.repo.nii.ac.jp:00012907,
 author = {指籏, 和也 and 市川, 麗子 and 茂木, 和枝 and 豊泉, 龍児},
 journal = {Science Journal of Kanagawa University},
 month = {Jun},
 note = {Rotifers (species belonging to the phylum Rotifera) have numerous motile cilia on their surface, and they use these cilia for locomotion and feeding. Especially, the corona, the most-anterior paired apparatus, has ciliary bands with densely packed long cilia. From zoological aspects, microscopic rotifers are intriguing organisms. Philodina sp. (class Bdelloidea, order Bdelloida) is well-known for its cryptobiosis, and this rotifer species has a potential to become a model organism for ciliary movement, because we can easily resuscitate Philodina by immersing it in artificial fresh water. The corona of the rotifer has two important ciliary bands, preoral ciliary one and postoral ciliary one, both of which are essential for swimming and feeding. However, mechanisms for the metachrony (metachronal wave), the coordinated ciliary movement of its corona, have not yet to be unveiled. In this report, ciliary beating of rotifer Philodina sp. was observed with a high-speed camera after treating it with the chemical agent, to investigate the role and mechanism of metachrony in the paired corona of the Philodina sp. By treating Philodina with Sodium Orthovanadate (V) (an inhibitor of dynein ATPase), trajectories of its swimming changed markedly compared with the control group; Philodina changed its swimming direction more frequently to form tiny circular trajectories. By treating Philodina with NiCl2 (an inhibitor of dynein ATPase), metachrony was disrupted and ciliary movement was mostly arrested. Neurotransmitters are known to be involved in the control of ciliary beating in several invertebrate species. Therefore, we next examined the effects of octopamine, norepinephrine (noradrenaline), and serotonin on the ciliary beating and locomotive behaviors of the Philodina sp. After the administration of octopamine or norepinephrine, confusion of metachrony and resultant disorder of the "collective V-shaped waveform" of metachrony were markedly induced. As the results, trajectories of swimming significantly increased the ratio of topical spiral circle lines. At higher dose of serotonin, the cilia movement of the preoral ciliary band was weakened or stopped, and frequent changes between ciliary locomotion and muscular creeping behavior were induced. The administration of nepicastat, an inhibitor of the biosynthesis of octopamine or norepinephrine, elicited retraction and closure of the paired corona into the body, and often caused the failure of creeping movement. These above changes may have been the reflection of the neurotransmitter-dependent control of metachronal ciliary movement of Philodina sp. of the bdelloid rotifer. In this report, we challenge to investigate the complex and fascinating ciliary metachrony of the Philodina rotifer. We hope that our efforts will promore studies on ciliary metachronal wave using the rotifer., Departmental Bulletin Paper, 原著},
 pages = {55--61},
 title = {輪形動物ヒルガタワムシの運動における繊毛冠の役割の研究},
 volume = {30},
 year = {2019}
}