{"created":"2023-05-15T11:50:46.122214+00:00","id":12536,"links":{},"metadata":{"_buckets":{"deposit":"d6276210-ff70-4a7d-add6-fb6a204e73d8"},"_deposit":{"created_by":10,"id":"12536","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"12536"},"status":"published"},"_oai":{"id":"oai:kanagawa-u.repo.nii.ac.jp:00012536","sets":["376:377:378:1219"]},"author_link":[],"item_3_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Purification and Characterization of Crustacean Hyperglycemic Hormone-Family Peptides with Vitellogenesis-Inhibiting Activity from the Southern Rough Shrimp Trachysalambria curvirostris"}]},"item_3_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"82","bibliographicPageStart":"77","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"Science Journal of Kanagawa University","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the southern rough shrimp Trachysalambria curvirostris , two CHH-family peptides were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The vitellogenesis- inhibiting activities of the CHH-family peptides were examined using ex vivo incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus . The two CHH-family peptides reduced vitellogenin mRNA levels in the ovarian fragments. The two CHH-family peptides did not show any hyperglycemic activity based on an in vivo injection assay. From these results, the two CHH-family peptides were designated as Trc-VIH-I and -II, respectively. Two cDNAs encoding Trc-VIH-I and -II precursors were cloned by RT-PCR coupled with 5'- and 3'-RACE. The mature Trc-VIH-I and -II were found to consist of 72 amino acid residues containing six conserved cysteine residues and possess an amidated C-terminus, respectively. The mature Trc-VIH-I and -II exhibited the highest sequence similarity with a CHH-family peptide showing vitellogenesis-inhibiting activity in a penaeid shrimp species, Litopenaeus vannamei VIH (Liv-SGP- G), by tBLASTn analysis. The mature Trc-VIH-I and -II shared amino acid identities of 63.0 and 74.3% with Liv-SGP-G, respectively. These results indicate that Trc- VIH-I and -II regulate vitellogenesis in T. curvirostris .","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"2017年度神奈川大学総合理学研究所共同研究助成論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川大学総合理学研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Research Institute for Integrated Science, Kanagawa University","subitem_publisher_language":"en"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12068302","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-0483","subitem_source_identifier_type":"PISSN"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"花塚, 真史","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"梶, 圭祐","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"吉田, 幸宏","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"筒井, 直昭","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"大平, 剛","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-31"}],"displaytype":"detail","filename":"13 サルエビ卵黄形成抑制ホルモンの精製および構造決定.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"13 サルエビ卵黄形成抑制ホルモンの精製および構造決定","url":"https://kanagawa-u.repo.nii.ac.jp/record/12536/files/13 サルエビ卵黄形成抑制ホルモンの精製および構造決定.pdf"},"version_id":"64699186-f6c8-4a9e-8162-5d55ed2dbae6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"crustacea","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crustacean hyperglycemic hormone-family peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vitellogenesis-inhibiting hormone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sinus gland","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"southern rough shrimp","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Trachysalambria curvirostris","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"サルエビ卵黄形成抑制ホルモンの精製および構造決定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"サルエビ卵黄形成抑制ホルモンの精製および構造決定","subitem_title_language":"ja"},{"subitem_title":"Purification and Characterization of Crustacean Hyperglycemic Hormone-Family Peptides with Vitellogenesis-Inhibiting Activity from the Southern Rough Shrimp Trachysalambria curvirostris","subitem_title_language":"en"}]},"item_type_id":"3","owner":"10","path":["1219"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-06-07"},"publish_date":"2019-06-07","publish_status":"0","recid":"12536","relation_version_is_last":true,"title":["サルエビ卵黄形成抑制ホルモンの精製および構造決定"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-12-11T06:47:51.593551+00:00"}