@article{oai:kanagawa-u.repo.nii.ac.jp:00012536,
 author = {花塚, 真史 and 梶, 圭祐 and 吉田, 幸宏 and 筒井, 直昭 and 大平, 剛},
 journal = {Science Journal of Kanagawa University},
 month = {Jun},
 note = {Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the southern rough shrimp Trachysalambria curvirostris , two CHH-family peptides were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The vitellogenesis- inhibiting activities of the CHH-family peptides were examined using ex vivo incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus . The two CHH-family peptides reduced vitellogenin mRNA levels in the ovarian fragments. The two CHH-family peptides did not show any hyperglycemic activity based on an in vivo injection assay. From these results, the two CHH-family peptides were designated as Trc-VIH-I and -II, respectively. Two cDNAs encoding Trc-VIH-I and -II precursors were cloned by RT-PCR coupled with 5'- and 3'-RACE. The mature Trc-VIH-I and -II were found to consist of 72 amino acid residues containing six conserved cysteine residues and possess an amidated C-terminus, respectively. The mature Trc-VIH-I and -II exhibited the highest sequence similarity with a CHH-family peptide showing vitellogenesis-inhibiting activity in a penaeid shrimp species, Litopenaeus vannamei VIH (Liv-SGP- G), by tBLASTn analysis. The mature Trc-VIH-I and -II shared amino acid identities of 63.0 and 74.3% with Liv-SGP-G, respectively. These results indicate that Trc- VIH-I and -II regulate vitellogenesis in T. curvirostris ., 原著, 2017年度神奈川大学総合理学研究所共同研究助成論文},
 pages = {77--82},
 title = {サルエビ卵黄形成抑制ホルモンの精製および構造決定},
 volume = {29},
 year = {2018}
}