{"_buckets": {"deposit": "d6276210-ff70-4a7d-add6-fb6a204e73d8"}, "_deposit": {"created_by": 10, "id": "12536", "owners": [10], "pid": {"revision_id": 0, "type": "depid", "value": "12536"}, "status": "published"}, "_oai": {"id": "oai:kanagawa-u.repo.nii.ac.jp:00012536", "sets": ["1219"]}, "author_link": [], "item_3_alternative_title_20": {"attribute_name": "その他の言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Purification and Characterization of Crustacean Hyperglycemic Hormone-Family Peptides with Vitellogenesis-Inhibiting Activity from the Southern Rough Shrimp Trachysalambria curvirostris"}]}, "item_3_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-06-30", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "82", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "29", "bibliographic_titles": [{"bibliographic_title": "Science Journal of Kanagawa University", "bibliographic_titleLang": "en"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the southern rough shrimp Trachysalambria curvirostris , two CHH-family peptides were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The vitellogenesis- inhibiting activities of the CHH-family peptides were examined using ex vivo incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus . The two CHH-family peptides reduced vitellogenin mRNA levels in the ovarian fragments. The two CHH-family peptides did not show any hyperglycemic activity based on an in vivo injection assay. From these results, the two CHH-family peptides were designated as Trc-VIH-I and -II, respectively. Two cDNAs encoding Trc-VIH-I and -II precursors were cloned by RT-PCR coupled with 5\u0027- and 3\u0027-RACE. The mature Trc-VIH-I and -II were found to consist of 72 amino acid residues containing six conserved cysteine residues and possess an amidated C-terminus, respectively. The mature Trc-VIH-I and -II exhibited the highest sequence similarity with a CHH-family peptide showing vitellogenesis-inhibiting activity in a penaeid shrimp species, Litopenaeus vannamei VIH (Liv-SGP- G), by tBLASTn analysis. The mature Trc-VIH-I and -II shared amino acid identities of 63.0 and 74.3% with Liv-SGP-G, respectively. These results indicate that Trc- VIH-I and -II regulate vitellogenesis in T. curvirostris .", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_3_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "原著", "subitem_description_language": "ja", "subitem_description_type": "Other"}, {"subitem_description": "2017年度神奈川大学総合理学研究所共同研究助成論文", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_3_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "神奈川大学総合理学研究所", "subitem_publisher_language": "ja"}, {"subitem_publisher": "Research Institute for Integrated Science, Kanagawa University", "subitem_publisher_language": "en"}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12068302", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1880-0483", "subitem_source_identifier_type": "PISSN"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "花塚, 真史", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "梶, 圭祐", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "吉田, 幸宏", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "筒井, 直昭", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "大平, 剛", "creatorNameLang": "ja"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-05-31"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13 サルエビ卵黄形成抑制ホルモンの精製および構造決定.pdf", "filesize": [{"value": "1.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1500000.0, "url": {"label": "13 サルエビ卵黄形成抑制ホルモンの精製および構造決定", "url": "https://kanagawa-u.repo.nii.ac.jp/record/12536/files/13 サルエビ卵黄形成抑制ホルモンの精製および構造決定.pdf"}, "version_id": "64699186-f6c8-4a9e-8162-5d55ed2dbae6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "crustacea", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "crustacean hyperglycemic hormone-family peptide", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "vitellogenesis-inhibiting hormone", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sinus gland", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "southern rough shrimp", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Trachysalambria curvirostris", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "サルエビ卵黄形成抑制ホルモンの精製および構造決定", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "サルエビ卵黄形成抑制ホルモンの精製および構造決定", "subitem_title_language": "ja"}, {"subitem_title": "Purification and Characterization of Crustacean Hyperglycemic Hormone-Family Peptides with Vitellogenesis-Inhibiting Activity from the Southern Rough Shrimp Trachysalambria curvirostris", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "10", "path": ["1219"], "permalink_uri": "http://hdl.handle.net/10487/00015643", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-06-07"}, "publish_date": "2019-06-07", "publish_status": "0", "recid": "12536", "relation": {}, "relation_version_is_last": true, "title": ["サルエビ卵黄形成抑制ホルモンの精製および構造決定"], "weko_shared_id": -1}
http://hdl.handle.net/10487/00015643
http://hdl.handle.net/10487/00015643