Two ferredoxins, FdA and FdB, were purified from the photosynthetic bacterium Heliobacillus mobilis . Their absorption spectra were typical of the 2[4Fe-4S] cluster with absorption peaks at 385 and 280 nm and with a shoulder at 305 nm. Fd-B was sensitive to oxygen, and its A385 values decreased by about 50% in 2 hours at 4℃ under aerobic conditions. In contrast, A385 of Fd-A remained unchanged for up to 24 h under the same conditions. Cryogenic ESR measurements indicated that Fd-A had ESR signals around g = 2.05, 1.94 and Fd-B around g = 2.05, 1.92. Both ferredoxins were fully reduced by dithionite at pH 8.0.
内容記述
短報
2017年度神奈川大学総合理学研究所共同研究助成論文
雑誌名
Science Journal of Kanagawa University
巻
29
ページ
93 - 96
発行年
2018-06-30
ISSN
1880-0483
書誌レコードID
AA12068302
著者版フラグ
publisher
その他の言語のタイトル
Analysis of Metal Proteins and Metal Complexes by Cryogenic ESR －Characterization of Ferredoxins from the Photosynthetic Bacterium Heliobacillus mobilis－
極低温ESRによる金属タンパク質及び金属錯体の機能解析 －光合成細菌Heliobacillus mobilisのフェレドキシンの性質－
16 極低温ESRによる金属タンパク質及び金属錯体の機能解析 －光合成細菌Heliobacillus mobilisのフェレドキシンの性質－
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